Part:BBa_K1151001:Experience

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User Reviews

UNIQ70c64be116d45a51-partinfo-00000000-QINU UNIQ70c64be116d45a51-partinfo-00000001-QINU

• Imperial College 2014

We attempted to use this protein as part of fusions with cellulose binding domains. However after cloning our fusions using this part we noticed that unfortunately this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. Although the sequence updated by the authors here corresponds to the actual DNA sequence of the part, the sequence itself is erroneous as it contains a deletion with respect to the original Heliobacter pylori NiBP (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). To correct the mutation a 'C' needs to be added between bases 52-53. This should be able to be easily achieved using site-directed mutagenesis PCR.

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